Optimizing a method of establishing colon cancer liver metastasis models

doi:10.3969/j.issn.2095-4344.2014.36.020

Abstract

Abstract:

BACKGROUND: The metastasis ways of colon cancer include blood metastasis, peritoneal metastasis and lymph node metastasis. Among them, the most common metastasis organ is the liver. It is necessary to establish a simple, suitable animal model to study the biological behavior of the colon cancer liver metastasis, the heterogeneity of cancer cells, and the mechanism of tumor metastasis.
OBJECTIVE: To establish animal model of colon cancer liver metastasis with splenectomy injection method.
METHODS: Human colon cancer cell lines HT29 were cultured, and subcutaneously transplanted tumor was produced in BALB/c mice. Models of colon cancer liver metastases were established through spleen injection and excision of spleen method. The tumor growth and metastasis, the pathology of transplanted tumor, DNA content and cell cycle of the transplanted tumor were all determined.
RESULTS AND CONCLUSION: In this study, we successfully established the model of colon cancer liver metastases through the spleen injection via the dorsal midline incision, the tumor formation rate reached 8/8 (100%). Observation of tissue slice showed that the transplanted tumor was adenocarcinoma I-II. Flow cytometry analysis results showed that DNA ploid was heteroploid DNA. This model provides a good technical platform for biology research for colon cancer metastasis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: colonic neoplasms, neoplasm metastasis, models, animal, splenectomy

CLC Number:

R318

Wang Li, Wen Xi-nian, Gao Hua, Xu Xin-cai, Li Tao, Zhang Wen-bin. Optimizing a method of establishing colon cancer liver metastasis models[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(36): 5855-5860.

Figures/Tables 3

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